ADHFE1 and C-MYC Association and Over-Expression in Different Stages of Human Breast Cancer Tissues


  • Sarah Sadiq, Umara Asim, Naseem Arshad, Ambreen Javed, Rabia Sadiq, Rabia Hamid, Ambreen Sadiq



Breast cancer, c-Myc, ADHFE-I and Immunohistochemistry.


Chief enantiomer in breast tumors is D-2HG. Mitochondrial Alcohol Dehydrogenase (ADHFE I ) produces D-2HG. lt is the CA breast oncogene which leads to decreased patient survival. c-MYC can upregulate ADHFE I by changes in iron metabolism whereas co expression of c-MYC and ADHFE I greatly augment ort hotopic growth of tumor in MCF7 cells.

Aims: To determine the expression levels and localization of c-M yc and ADHFE I in different stages of breast cancer tissue.

Study Design: Cross-sectional comparative study.

Methodology: Present study was conducted to enhance knowledge of c-Myc function in breast cancer by examination of relation between nuclear c-Myc and iron containing ADHFE I enz yme protein expression, using immunohist cichemistry. All this information was recorded on performa.

Statistical analysis: Data was analyzed using SPSS version 25. Analysis of variance (ANOVA) test, was employed to observe mean difference between groups.

Results: Nuclear c-Myc and cytoplasmic ADHFE I levels were significantly higher than controls and were positively correlated with each other in advancing stages of breast cancer.

Conclusion:  It was concluded that the association between the expression levels of c-Myc oncogene and ADHFE-1 enzyme existed in all advancing stages of CA Breast and the expression of both increased as the breast cancer advanced.